Isopropanol precipitation removes PCR inhibitors from ancient bone extracts
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چکیده
منابع مشابه
Ethanol re-precipitation removes PCR inhibitors from Ancient DNA extract
One of the major problems in ancient DNA work is the presence of inhibitory substances, which hampers Taq polymerase activity. Therefore analysis of ancient DNA sample is very challenging. Here we describe a simple and competent ethanol re-precipitation based protocol for the purification of DNA from ancient bones and tissues. The efficiency of this procedure has been demonstrated on 600 years ...
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was detected after electrophoresis.This result is correlated with the fact that this tissue does not express this gene (data not shown). Semi-solid-phase cDNA libraries were shown to be representative of rare tissue mRNAs, as the number of neurons expressing MCH, DYN or SgII is small in our samples: about 30 cells expressing the MCH gene could be counted in representative samples. Furthermore, ...
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A simple and effective modified ethanol precipitation-based protocol is described for the preparation of DNA from ancient human bones. This method is fast and requires neither hazardous chemicals nor special devices. After the powdering and incubating of the bone samples Dextran Blue was added as a carrier for removing the PCR inhibitors with selective ethanol precipitation. This method could e...
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Severe contamination of electroencephalographic (EEG) activity by eye movements, blinks, muscle, heart and line noise is a serious problem for EEG interpretation and analysis. Rejecting contaminated EEG segments results in a considerable loss of information and may be imLractical for clinical data. Manv methods have been proposed to remove eye movement and blink" artifacts from EEG recordings. ...
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A method to measure apolipoprotein B radioactivity in whole blood plasma is described that is suitable for routine use in kinetic experiments in vivo. Radiolabeled apolipoprotein B is precipitated from plasma diluted 15- to 30-fold in the presence of carrier low density lipoproteins by 50% isopropanol. The amount of radioiodine in apoB is estimated from the difference between total radioiodine ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1995
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/23.5.881